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How to Build a Closed Freshwater Biosphere

A biosphere is a contained, self-sufficient system of living organisms that interact with each other and the natural environment to carry out the cycles of life. A pond is an example of a freshwater biosphere that can be scaled down in size to fit inside a sealed container for scientific study. By bottling a sample of pond water, small invertebrates, bacteria, plants, and mud, you can create a self-sustaining ecosystem where you can observe aquatic creatures thriving in their natural surroundings.

Things You'll Need

  • Clean bottle, jar, or other clear glass container that can be sealed completely
  • Airtight lid for the container
  • Hammer
  • Small nail
  • Candle
  • Match
  • Pond water and mud (naturally contains tiny invertebrates and bacteria)
  • Small pond plants
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Instructions

    • 1

      Hammer the nail through the lid of the container to make a small hole, then light the candle and melt a drop of wax onto the hole that will form a seal when it cools. This will act as a safe pressure outlet to prevent explosion if gases build up inside the container.

    • 2

      Add mud to the container, filling to about 2 inches deep. Pour in pond water until the container is about two-thirds full.

    • 3

      Add about 1/4 cup of algae and small plants. Don't add large plants, fish, crustaceans, or insects that are found above the pond's surface -- they will more likely die in a small contained environment.

    • 4

      Place the lid on the container and seal it completely. Do not open it again until you are ready to discard the biosphere. The plants and animals inside the container will survive by making their own oxygen, carbon dioxide, and food within the closed system.

    • 5

      Place the biosphere container indoors where it will receive partial sunlight throughout the day. Direct sunlight can cause the temperature inside the container to fluctuate too dramatically.

    • 6

      Observe changes in the biosphere over the course of days, weeks, and even months. If plants die and the biosphere seems inactive, empty the container, wash it thoroughly and repeat the above steps to create a new biosphere.

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