* Akbar (born 1542): The most famous of his children, he became the Mughal emperor after Humayun's death.
* Hami (born 1534): Hami died young.
* Mirza Muhammad Hakim (born 1537): Ruled Kabul and Kandahar for some time.
* Mirza Abdur Rahim (born 1540): Humayun's fourth son, died young.
* Mirza Daniyal (born 1542): Humayun's youngest son, died in 1604.