How to Calibrate an Autoclave

Timer Calibration

• 1

  Set the timer on the autoclave for a full cycle. Hold the stopwatch and be prepared to click it.

• 2

  Turn on the autoclave and the stopwatch simultaneously.

• 3

  Repeat the process if the time and the stopwatch do not match.

• 4

  If the autoclave timer differs from the stopwatch time by more than 30 seconds, post an appropriate correction factor directly on the autoclave.

• 5

  Note the calibration results in a logbook.

Temperature Calibration

• 6

  Place the maximum registering thermometer near the built-in temperature sensor.

• 7

  Load and operate the autoclave as usual.

• 8

  Record the maximum autoclave temperature according to the built-in temperature display.

• 9

  Let the autoclave cool.

• 10

  Remove the maximum registering thermometer from the autoclave and record the temperature reading in the logbook.

• 11

  Record the discrepancy if the maximum temperature of the built-in display is different from the maximum temperature on the registering thermometer.

• 12

  Post a correction factor on the autoclave control panel if the temperatures were different.

Pressure Calibration

• 13

  Calibrate the timer and temperature gauges before these steps.

• 14

  Load and operate the autoclave as usual. When the temperature display shows 121 degrees Celsius, the pressure gauge should show 15 pounds per square inch (15 psi).

• 15

  Record the discrepancy if there is any.

• 16

  Mark the correct point for 15 psi on the cover of the gauge and post a correction factor on the autoclave if this is not true.