Use a Bunsen burner to sterilize the petri dish culture. The flame should be 20 centimeters from the dish and held there for 20 seconds.
Use a marker to draw a "T" at the bottom of the agar streak plate, dividing it into three sections.
Hold the inoculating loop near the Bunsen burner until it turns red.
Lift up the lid of the petri dish and insert the inoculating loop, pushing it to the side of the dish.
Use the inoculating loop and touch an edge of a colony near the center of the petri dish.
Take out the inoculating loop and close the dish.
Open the agar streak plate and drag the point of inoculating loop to the largest section of the plate, but not the part marked with marker.
Drag the inoculating loop, in a winding motion back and forth, from the large section to each of the smaller boxes.
Replace the lid of the agar dish with a new lid and seal it with a layer of parafirm. The bacteria is then ready for the incubation process.