How to Make Chitosan

Chitosan is a chemical byproduct found in chitin produced by shellfish. It is touted to have antibacterial properties and is used in beauty products, weight loss products and bandages. Chitin is extracted from shellfish using common chemicals found in a laboratory, under careful watch. Before you extract chitin, ensure you have proper safety gear such as a lab coat, rubber gloves and goggles to protect your skin and eyes. Some of the chemicals used are caustic.

Things You'll Need

  • 10 percent sodium hydroxide
  • Petri dish
  • Bunsun burner
  • 1 percent hydrochloric acid
  • Sodium hydroxide
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Instructions

    • 1

      Extract chitin from your shellfish by diluting your shell in 10 percent sodium hydroxide and heat to 80 to100 degrees. This will make the shell water-soluble. Chitin is a mineral compound found in the shell. Once it is soaked and heated it will look like fine grains, identifiable under a microscope.

    • 2

      Remove the calcium carbonate by allowing the chitin to soak in a petri dish of 1 percent hydrochloric acid at room temperature for 10 to 15 minutes. Calcium carbonate will dissolve completely, leaving behind the chitin.

    • 3

      Dissolve your chitin in less than 40 percent sodium hydroxide. Heat to a temperature of 90 to 120 degrees with a bunsun burner. This removes 65 percent of the acetylic groupings to allow them to dissolve during preparation, forming the chitin into chitosan.

    • 4

      Mix chitosan with deionized water, matching the weight to the amount of water required. This will neutralize the chitosan and prepare it for use. When using chitosan for makeup production, do not weaken with sodium hydroxide.

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