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How to Resuspend Oligos

Primer sequences for experiments in molecular biology are short strands of nucleotides, typically 18 to 24 nucleotides in length. These are called oligonucleotides (from the Greek word "oligo," meaning "few") and are dehydrated prior to shipping. Resuspend your oligos (as they are commonly called) initially in either 1X TE buffer or sterile water (autoclaved dH2O) to make a 100μM concentration. This is your stock solution that is used to make other dilutions.

Centrifuge
Vortex mixer
Sterile dH2O or 1X TE buffer

Instructions

- 1
  Centrifuge your tubes before opening, to make sure pellets are at bottom of the tube. These pellets are usually too small to see with the naked eye.
- 2
  Read the specifications sheet that comes with your oligos. For each oligo, the shipper will provide you with the amount of your primers in nanomoles. Multiply this number by 10 for the amount of solvent to add to each tube to make a 100μM stock concentration. For example, to a primer quantity of 37.25nm, you would add 372.5μL of TE or dH2O.
- 3
  Mix solution by briefly vortexing.
- 4
  Note the "100μM" concentration on the tube with the day's date.

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