Western blotting is a common technique in the biological sciences for detecting proteins using gel electrophoresis to separate polypeptides according to length. The relative amounts of a protein present in different samples and the protein's molecular weight can be detected with a Western blot. The results are transferred to blotting paper (nitrocellulose), then incubated with a generic protein, an antibody and a colorless substrate that will be converted to a colored substrate by an enzyme attached to the antibody. The length of time the blot can be stored depends on the stability of the proteins.
Instructions
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1
Dry the blot. Sandwich the blot between two clean sheets of filter paper.
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2
Place the blot and filter paper between two sheets of card stock.
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3
Place paper clips around the edges of the stack. Do not overlap the blot with the paper clips.
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4
Place the paper-clipped stack into a sealable plastic bag, then seal the bag.
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5
Store the blot at a low temperature if possible for best results. Generally, you can store the blot at 4 degrees Celsius for up to 2 weeks, -20 degrees Celsius for up to 2 months and -70 degrees Celsius if longer term storage is needed.