Steps to Fix Low Power Under a Compound Microscope

Learning how to "fix" low power, or rather how to focus under low power, is an important step in identifying a sample on a compound microscope. Focusing should begin at the lowest objective power using the coarse knob. Once this has been achieved, the user can move to the next highest objective power and focus the sample a little more clearly. At the highest power only the fine adjustment knob is used for small focus changes. When done correctly, focusing at the lowest objective power can allow for a faster progression up to the highest power.

Instructions

    • 1
      Moving the sample around to where part of it is visible through the eyepiece should help make locating the sample at higher powers easier.

      Place the microscope slide onto the mechanical stage where the sample on the slide can be seen through the ocular lens. The mechanical stage is the platform right below the objectives. At this point the specimen does not have to be in focus, and it may appear as a blurry blob. This is OK as long as part of the blurry blob can be seen through the ocular lens.

    • 2
      The coarse adjustment knob is located on and closest to the microscope arm. The knob that sticks farther out is called the fine adjustment knob.

      Use the coarse adjustment knob to bring the blurry blob into focus. The coarse adjustment knob is located at the bottom of the microscope arm, just before the microscope base. It will be a a set of two knobs combined into one: a smaller knob on the outside, which is the fine adjustment knob, and a larger ring knob directly against the microscope arm. This larger ring knob is the coarse adjustment knob, which is good for large focus corrections on lower objective powers.

    • 3
      Too much light can bleach out the sample image seen through the eyepiece; too little and it can be hard to differentiate between the sample and the slide background.

      Adjust the light source so the right amount of light passes through the sample and makes the best contrast with the microscope slide background. The light source is usually located on the microscope base, either as a knob on the front of the microscope base, or as a partial knob for turning located on the side of the microscope base.

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