The protagonist of the story. A high school student who gained superhuman abilities after being bitten by a radioactive spider. He uses his powers to fight crime and protect the city of New York as the masked superhero Spider-Man.
Gwen Stacy:
Peter Parker's love interest and classmate. She is intelligent, compassionate, and supportive of Peter's superhero activities.
Harry Osborn:
Peter's best friend and the son of Norman Osborn, the CEO of Oscorp Industries. He initially struggles with jealousy towards Peter but eventually comes to terms with their friendship.
Dr. Curtis Connors / The Lizard:
A brilliant scientist who becomes a monstrous reptilian creature called the Lizard after an experiment gone wrong. He seeks to transform all of humanity into lizards and poses a significant threat to the city.
Captain George Stacy:
Gwen's father and a police captain who is determined to capture Spider-Man, believing him to be a menace. He later sacrifices himself to save Peter and Gwen.
Norman Osborn / The Green Goblin:
The ruthless and power-hungry CEO of Oscorp Industries. He becomes the villain Green Goblin after an experiment with an unstable performance enhancer alters his personality. He seeks revenge against Spider-Man and threatens the city with his advanced technology and destructive glider.
Dr. Otto Octavius / Doctor Octopus:
A renowned physicist who turns into the villain Doctor Octopus after an accident fuses four powerful mechanical arms to his body. He seeks to harness unlimited energy and becomes obsessed with proving his scientific prowess.
Mr. Bernard:
Peter Parker's uncle who raises him after the death of his parents. He provides Peter with guidance, wisdom, and support.