Divide the final count by the initial countld. For example, if you had 100 bacteria to start and an hour later had 80 bacteria, you would divide 80 by 100 to get 0.8.
Use the calculator to take the natural log (often abbreviated "ln" on calculators) of the result from Step 1. In this example, you would take the natural log of 0.8, which equals -0.223143551.
Divide the result from Step 2 by 2 to calculate the rate of decay. In this example, you would divide -0.223143551 by 2 to get a rate of decay of -0.111571776.