Here are a few ways the term *might* be used informally and incorrectly:
* Referring to the female friends or significant others of fraternity members: Someone might colloquially refer to a fraternity member's girlfriend or close female friend as their "little sister," but this is purely a friendly term, not an official title or role.
* A misinterpretation of a philanthropy event: Some fraternities might partner with organizations that support young women, and someone might mistakenly use the term "little sisters" in that context. This is again an inaccurate use of the term.
In short, the concept of "little sisters" doesn't exist within the formal structure of a fraternity. If you hear the term used, it's likely a casual and informal reference with no official meaning.