Use brassica plants grown from aseptic seed germination (seeds that have undergone a sterilization procedure prior to germination), or plants that have undergone specific sterilization protocols and been tested for lack of microbial contamination.
Wash your hands and wipe them with 70 percent ethanol, then apply gloves. Wipe fume hood surfaces with 70 percent ethanol. Place the petri dish containing agar, supplemented with nutrients, into the fume hood after wiping the upper and lower surface of the dish with 70 percent ethanol.
Place the plant between two sheets of sterile paper towels. Cut a five-to-10 millimeter piece of plant from any part of the plant with the scalpel. Keep the scalpel and forceps in the beaker of ethanol when not in use.
Use the forceps to place the cut piece of plant into the sterile petri dish and press the cut end into the nutrient-rich agar.
Incubate the transplants in a growth chamber or greenhouse until rooted.