* Trust and betrayal: The relationships between the main characters, and their evolving trust (or lack thereof) in each other and authority figures.
* The power of science: The novel highlights both the incredible potential and the inherent dangers of scientific progress, specifically in genetic research.
* Coming of age: The protagonists are teenagers navigating complex situations and making important decisions about their future and their values.
* Family and loyalty: The importance of family bonds, both biological and chosen, are explored throughout the narrative.
While the mystery plot itself is a significant component, the underlying message concerning the ethical responsibilities of scientific innovation forms the dominant theme.