The Babington Letter contained several incriminating statements, including:
- A declaration of Babington's loyalty to Mary and his willingness to sacrifice his life for her cause
- A detailed plan for the assassination of Queen Elizabeth, involving a group of six men who would attack the queen and her guards
- A request for Mary's approval and support for the plot
- A list of Catholic noblemen and gentlemen who were said to be involved in the conspiracy
- A proposal for a foreign invasion of England to support Mary's claim to the throne
The Babington Letter provided clear evidence of Mary's involvement in a plot to overthrow Queen Elizabeth, and it was used as a key piece of evidence in her trial and subsequent execution in 1587.