EMS Mutagenesis Protocol

Mutagenesis protocols are processes used to cause permanent gene mutations. Genes mutate on a regular basis, depending on the current demands of the environment; however, few of these opportunistic mutations are passed on. In order to create stable, controlled mutations, protocols are used. According to the American Society of Plant Biologists, the EMS has a point mutation effect, which is a direct change in the genetic alphabet of a gene.
  1. Function

    • Ethyl Methyl Sulfonate Is Toxic

      The chemical compound ethyl methyl sulfonate (EMS) is used in laboratory research to mutate plants, yeasts and other organisms. EMS is a highly carcinogenic substance, and researchers have to use fume hoods and decontaminate the working area carefully.

    Benefits of Protocols

    • Mutagenesis protocols are very complex.

      The mutagenesis protocols consist of complex, timed, repeated processes. Through experimentation, scientists have produced methods of achieving certain results. For example, the Cook Laboratory of the University of California uses a special EMS protocol to mutate the seeds of the plant M. truncatula, while the Cold Spring Harbor Laboratory in New York has developed an EMS protocol for yeast.

    Protocols Are Properties

    • Scientists have an eye to marketing their research.

      Protocols are legal properties of the people and entities who created them. Research on mutagenesis may lead to valuable, permanent traits in plants and organisms that can be marketed.

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