Specifically, it involves the recombination of gene segments (V, J, and sometimes Cκ) within the kappa light chain locus on chromosome 2 in humans (chromosome 6 in mice). This recombination process, mediated by the RAG1 and RAG2 recombinases, creates a unique variable (V) region for the kappa light chain. This V region is essential for the antibody's antigen-binding specificity. If successful, the rearranged kappa gene is transcribed and translated to produce a kappa light chain protein. If unsuccessful, the cell may attempt to rearrange the lambda light chain gene instead. Only cells that successfully rearrange either a kappa or lambda light chain are able to complete the development into mature B cells.