Marvel:
* Iceman: Bobby Drake, also known as Iceman, is a mutant with the ability to control ice and cold. He is one of the original members of the X-Men and has been a prominent member of the team for many years. Iceman is gay and came out in 2015.
* Northstar: Jean-Paul Beaubier is a mutant with the ability to fly and emit powerful energy blasts from his eyes. He is a member of Alpha Flight, a Canadian superhero team. Northstar is gay and married his long-time partner, Kyle Jinadu, in 2012.
* America Chavez: America Chavez is a young Latina superhero with the ability to create star-shaped portals that allow her to travel through time and space. She is a member of the Young Avengers, a team of teenage superheroes. America Chavez is lesbian and is in a relationship with fellow Young Avenger, Kate Bishop.
* Loki: Loki is the Norse god of mischief and brother of Thor. He is a powerful sorcerer and has been both an enemy and an ally of the Avengers. Loki is genderfluid and has been portrayed as male, female, and non-binary.
* Poison Ivy: Poison Ivy is a supervillain and an enemy of Batman. She has the ability to control plants and toxins and uses them to commit crimes. Poison Ivy is bisexual and has been in relationships with both men and women.
DC:
* Batwoman: Kate Kane is a lesbian crime-fighter who operates in Gotham City. She is a highly skilled vigilante and uses her wealth to fund her crime-fighting activities. Batwoman is also a member of the Justice League.
* The Flash (Wally West): Wally West is the second Flash and the nephew of Barry Allen, the original Flash. Wally is a speedster with the ability to move and think at super-human speeds. He is also a member of the Justice League. Wally West is gay and married his long-time partner, Linda Park, in 2016.
* Midnighter: Midnighter is a superhero with enhanced strength, speed, and durability. He is also an expert martial artist and weapons specialist. Midnighter is gay and married to his long-time partner, Apollo.
* Apollo: Apollo is a superhero with the power of flight and super-human strength. He is also a skilled archer and swordsman. Apollo is gay and married to his long-time partner, Midnighter.
* Harley Quinn: Harley Quinn is a supervillain and an enemy of Batman. She is a former psychiatrist who fell in love with the Joker and became his sidekick. Harley Quinn is bisexual and has been in relationships with both men and women.